品牌 | SOLARBIO/索萊寶 | CAS | 詳詢 |
---|---|---|---|
分子式 | 詳詢 | 純度 | 詳詢 |
分子量 | 詳詢 | 貨號(hào) | BC1560 |
規(guī)格 | 50管/24樣 | 供貨周期 | 現(xiàn)貨 |
主要用途 | 谷草轉(zhuǎn)氨酶(GOT)活性檢測(cè) | 應(yīng)用領(lǐng)域 | 環(huán)保,化工,生物產(chǎn)業(yè),農(nóng)業(yè),綜合 |
試劑名稱 | 規(guī)格 | 保存條件 |
提取液 | 液體30 mL×1瓶 | 2-8℃保存 |
試劑一 | 粉劑×2瓶 | 2-8℃保存 |
試劑二 | 液體8 mL×1瓶 | 2-8℃保存 |
試劑三 | 液體80 mL×1瓶 | 2-8℃保存 |
標(biāo)準(zhǔn)液 | 液體1 mL×1支 | 2-8℃保存 |
注意:實(shí)驗(yàn)之前建議選擇2-3個(gè)預(yù)期差異大的樣本做預(yù)實(shí)驗(yàn)。如果樣本吸光值不在測(cè)量范圍內(nèi)建議稀釋或者增加樣本量進(jìn)行檢測(cè)。
需自備的儀器和用品:
可見分光光度計(jì)、水浴鍋、臺(tái)式離心機(jī)、可調(diào)式移液器、1mL玻璃比色皿、研缽/勻漿器/、冰和蒸餾水。
操作步驟:具體操作步驟詳見“產(chǎn)品資料"附件
實(shí)驗(yàn)實(shí)例:
取0.1g兔子肝臟組織加入1mL提取液進(jìn)行勻漿研磨,取上清后再按照測(cè)定步驟操作,測(cè)得A測(cè)定管=0.782, A對(duì)照管=0.488,標(biāo)準(zhǔn)曲線y=0.4767x+0.0611,計(jì)算?A=A測(cè)定管-A對(duì)照管=0.782-0.488=0.294,x=(0.294-0.0611)÷0.4767=0.489,按樣本質(zhì)量計(jì)算酶活得:
GOT活性(U/g 質(zhì)量)=12x÷W=12×0.489÷0.1=58.68 U/g質(zhì)量。
相關(guān)發(fā)表文獻(xiàn):
Yong Li, Fengjun Cao, Mingxing Li,et al. Hydroxychloroquine induced lung cancer suppression by enhancing chemo-sensitization and promoting the transition of M2-TAMs to M1-like macrophages. Journal of Experimental & Clinical Cancer Research. October 2018;(IF5.646)
Poopal R K, Zhang J, Zhao R, et al. Biochemical and behavior effects induced by diheptyl phthalate (DHpP) and Diisodecyl phthalate (DIDP) exposed to zebrafish[J]. Chemosphere, 2020: 126498.
參考文獻(xiàn):
Zhao W, Wei H. Effects of cadmium on transaminase activities and structures of tissues in freshwater giant prawn (Macrobrachium rosenbergii) [J]. Journal of Fisheries of China, 1995,19(001):21-27.
Ohgami N, Upadhyay S, Kabata A, et al. Determination of the activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in a microfluidic system[J]. Biosensors and Bioelectronics, 2007, 22(7): 1330-1336.
相關(guān)系列產(chǎn)品:
BC0180/BC0185 半*氨酸(Cys)含量檢測(cè)試劑盒
BC1580/BC1585 *氨酸(Glu)含量檢測(cè)試劑盒
BC0250/BC0255 羥*氨酸(HYP)含量檢測(cè)試劑
谷草轉(zhuǎn)氨酶(GOT)測(cè)試盒 氨基酸代謝 谷草轉(zhuǎn)氨酶(GOT)測(cè)試盒 氨基酸代謝